Fig. 1: Cryo-EM structure of TcMscS.
From: Lipid-mediated gating of a miniature mechanosensitive MscS channel from Trypanosoma cruzi
a Domain organization of TcMscS and EcMscS. b Cryo-EM reconstruction of the heptameric TcMscS channel. Shown are orthogonal views of the cryo-EM density map sharpened by deepEMhancer. Each subunit is in a unique color. c The overall structure. d Structure of a single subunit with each domain distinctly colored. e Surface representation (left: space-filling; right: electrostatic potential (red, − 10 kT/e; white, neutral; blue, + 10 kT/e) of TcMscS and EcMscS (PDB: 6RLD). The structures of TcMscS and EcMscS were aligned by the conserved soluble β-domain. The dashed lines demarcate the membrane boundaries indicated by cryo-EM structures of the full-length EcMscS. f All-atom MD equilibrated position of the wild-type TcMscS in a POPC bilayer. Membrane deformation is depicted by the density of lipid headgroups shown in gray. A single-channel subunit is colored. g Lipid coordinates averaged over 2 µs trajectory using MOSAICS76. Lipids are colored by the atom types (cyan carbon, red oxygen, blue nitrogen, and orange phosphorus). The channel is shown in gray.
