Fig. 4: Conformation comparison of SLC26A7 to other SLC26 homologs.

a Cluster analysis of SLC26s structures. Paired RMSD of experimental structures of SLC26s was calculated, based on which a heatmap was generated. Three major groups representing inward-facing, intermediate and outward-facing can be identified by color differences. The color codes correspond to RMSD (unit: Angstrom). SLC26A7 apo and substrate (iodide) binding state (highlighted by blue box) are classified in intermediate group. b–d Structure alignment of SLC26A7 with SLC26A9 (Inward-facing, PDB 7CH1), Prestin (intermediate, PDB 7LH2), and Pendrin (Outward-facing, PDB 7WLE) respectively. Gate domains are aligned to show core domain conformational change. Conformation of SLC26A7 core domain is well-aligned to Prestin (intermediate). e Cartoon scheme to present the structure alignment results in a simpler way. Only TM3 and TM10 are depicted.