Fig. 1: Cryo-EM structure of Net4ΔC-γ1ΔC.

a Different views of a color-coded cryo-EM map. b Analogous views of the molecular model. Net4ΔC, γ1ΔC, N-glycans, and disulfide bonds are colored in orange, blue, pink, and yellow, respectively. Different structural elements are labeled in the figure. c Four specifically N-glycosylated asparagine residues6,19 colocalize with four unique Coulomb densities representing N-glycans in the map confirming the correctness of the model. Seven N-Acetylglucosamine (NAG 1-7) moieties were included in the model.