Fig. 10: Modeling cortex folding in Cep83 tKO and Fgf10 tKO mice.
From: Cortex folding by combined progenitor expansion and adhesion-controlled neuronal migration
a Number of cells based on DAPI staining per cortical area in CTL and Cep83 tKO and Fgf10 tKO mice. b Scheme illustrating the position of the Flrt-positive (blue) and –negative (grey) particles. Lines between the particles indicate attraction forces that are random within a small range (ε). c, d Sinus equations representing the attraction forces among Flrt-positive (blue) and negative (grey) particles in both control, (c) and Flrt1/3 dKO condition, (d). In the latter, Flrt-negative particles show increased adhesion forces (higher amplitude) than Flrt-positive ones. Dashed lines indicate the small range of variation (ε). e–h Surface plots and line graphs of the distribution of the particles on the Z axis after computer simulations. The control situation with a 50:50 ratio of Flrt+ and Flrt-negative particles results in a uniform surface, (e). The Flrt1/3 dKO condition with altered attraction forces results in a wavy surface, (f). This effect is enhanced by increasing the number of particles and changing the proportions between Flrt-positive and -negative particles, g, h 2D plots representing the contour of a central slice through the surface plot are shown below. Deeper areas of the surface (below 0.5), mainly present in the (f, g) condition, are colored in blue. Elevated areas (above 1.2, representing gyri-like structures) are more prominent in the (h) condition; these are colored in red and marked with a red arrow. i Number of particles, surface area, and percentage of simulations with gyri-like areas (n = 25) calculated from conditions, (e–h). Data are represented as a box plot, with median (center line), interquartile range (box) and minimum and maximum values (whiskers). one-way ANOVA with Tukey’s post hoc analysis.
