Fig. 4: Cortex enlargement induced by Fgf10 and Flrt1/Flrt3 deletion.

a Representative Satb2 (green) and DAPI (blue) stained E17.5 rostral sections of Fgf10 lx/lx control and Fgf10 cKO mice. VZ ventricular zone, SVZ subventricular zone, IZ intermediate zone, CP cortical plate, MZ marginal zone. Scale bar, 100  μm. b Quantifications of cortical laminar thickness shown in (a). Measurements were obtained from the bottom of the apical surface of the VZ to the pial surface. Fgf10 lx/lx, n = 6 brains; Fgf10 cKO, n = 6 cortices from 3 brains from 4 litters. Data are represented as a box plot (as in Fig. 1). two-tailed t-test with Welch correction. c Representative E17.5 Foxg1-Cre;Fgf10 cKO brain section labeled with Satb2 (green), Ctip2 (red), and DAPI (blue). Area in dashed rectangle is shown with higher magnification on the right. Dashed line indicates a gyrus. Scale bars, 200 μm. d Representative Satb2 (green) and DAPI (blue) stained rostral sections of CTL and Flrt1/3 dKO mice. Scale bar, 100 μm. e Quantifications of cortical laminar thickness shown in (d). Measurements were taken as in (b). CTL n = 3 brains; Flrt1/3 dKO, n = 3 brains from 3 litters. Data are represented as a box plot (as in Fig. 1). two-tailed t-test with Welch correction. f Representative whole-mount images of E17.5 CTL, Fgf10 dKO and Fgf10 tKO brains. Dashed areas were measured to obtain quantifications in (g). Scale bar, 1 mm. g Quantifications of the cortical areas shown in (f). CTL, n = 8 brains; Fgf10 dKO, n = 22 brains; Fgf10 tKO, n = 13 brains from 11 litters. Data are represented as a box plot (as in Fig. 1). one-way ANOVA, Tukey Post hoc test. h Representative whole-mount images of E17.5 Fgf10 lx/lx and Fgf10 cKO brains. Dashed areas were measured to obtain quantifications in (i). Scale bar, 1 mm. i Quantifications of the cortical areas shown in (h). Fgf10 lx/lx, n = 21 brains; Fgf10 cKO, n = 24 brains from 5 litters. Data are represented as a box plot (as in Fig. 1). two-tailed t-test with Welch correction.