Fig. 8: Comparison of the adenosine receptor G protein interface.
From: Molecular basis of ligand binding and receptor activation at the human A3 adenosine receptor
A Comparison of adenosine receptor—Gα complexes. The view is of the interaction of Gα with the receptor. Proteins are coloured according to the legend. The red dashed box indicates regions shown in panels B-F. Structures shown and coloured as: A3AR:ADO:DNGi1 (coloured cyan and purple, PDB: 9EBH); A3AR:Piclidenoson:mGsi (coloured green and light purple, PDB: 9EBI); sheep A3AR:ADO:Gi1 (coloured blue and grey, PDB: 8YH2); A1AR:ADO:DNGi1 (coloured light green and dark green, PDB: 7LD4); A2AAR:NECA:mGs (coloured yellow and orange, PDB: 6GDG); and A2BAR:ADO:mGs (coloured light pink and red, PDB: 8HDP, orange). B, C Detailed view of the A3AR:G protein interface from B the adenosine-bound A3AR-DNGi1 complex and C the Piclidenoson-bound A3AR-mGsi complex. Receptor residues are shown in shades of green, and G protein residues are in shades of purple. Receptor residues are numbered according to Ballesteros and Weinstein numbering, and G proteins are numbered according to the CGN scheme. D Comparison of G protein coupling in different adenosine receptor structures, highlighting the positions of TM6 and the α5 helix. E Overall Comparison of the α5 helix orientation in different Gs and Gi protein-bound adenosine receptor structures. F Comparison of the αN helix from the adenosine-bound A3AR structure (purple) with other adenosine receptor:G protein complexes.
