Fig. 5: Antibody blockade of Basigin rescues GC-induced endothelial and skeletal impairments in vitro. | Nature Communications

Fig. 5: Antibody blockade of Basigin rescues GC-induced endothelial and skeletal impairments in vitro.

From: Basigin links altered skeletal stem cell lineage dynamics with glucocorticoid-induced bone loss and impaired angiogenesis

Fig. 5: Antibody blockade of Basigin rescues GC-induced endothelial and skeletal impairments in vitro.The alternative text for this image may have been generated using AI.

a Tube formation assay of human VeraVec endothelial cells treated with supernatant from different experimental groups. n = 6 independent replicates per group from two independent experiments. b Representative brightfield images of endothelial scratch assay (top) and its quantification displayed as boxplots (bottom) after 12 h. Endothelial cells were treated with supernatant of cultured hSSCs treated as shown. n = 6 independent replicates from two independent experiments for Ctrl+aBsg and Bsg-OE+aBsg groups. n = 12 independent replicates from three independent experiments for Bsg-OE group. Boxplots with box and whiskers Min to Max. c In vitro osteogenesis of patient-derived human SSCs. Left: Boxplots showing quantification of Alizarin Red S staining. Right: Representative images of Alizarin Red S staining. Ctrl: control media only; Ctrl+PTH: control media with PTH 1-34 treatment 6 h before media change; Ctrl+aBsg: control media with Basigin antibody treatment. Bsg-OE: Lentivirally Basigin-overexpressing human SSCs with control media. n = 10 independent replicates of cells from two independent donors. Data shown as mean ± SEM. Statistical testing in a,c by one-way ANOVA with Fisher-LSD test, in b by Wilcoxon signed-rank test. *p < 0.05, **p < 0.01 ***p < 0.001, ****p < 0.0001. Scale bars, 100 µm.

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