Fig. 5: CryoEM of recombinant (rec) Paenibacillaceae F-ENA-2 fibers. | Nature Communications

Fig. 5: CryoEM of recombinant (rec) Paenibacillaceae F-ENA-2 fibers.

From: Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread family of endospore appendages across Firmicutes

Fig. 5

a CryoEM micrograph of rec-F-ENA-2 with corresponding 2D class average in (b); c nsTEM micrograph of rec-F-ENA-2 with corresponding 2D class averages in (d); e reconstructed cryoEM volume with (f) the corresponding molecular model shown in cartoon representation; g top-view slice-through along the C3 axis; h zoom-in of the beta-cylinder extension; i docking site of the N-terminal hook; j hydrophobic (left) and electrostatics (right) surface coloring; k structural overlay of the DUF4183 domains of F-ENA and F-ENA-2; l genetic loci of F-ENA-2 for two members of the Paenabillacaea group; and m AlphaFold3 (AF3) model of the F-ENA-2 and F-Anchor-2 complex. The images in (a, c) were derived from two biological replicates.

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