Fig. 3: In vitro incorporation assay.
From: The structure of the mammalian bornavirus polymerase complex
In vitro [α32P]-ATP incorporation assays for the 3′ extension activity of the L-P complex assessing elongation on a 13 or 14 nt template. Assays were performed in the presence of only [α32P]-ATP or [α32P]-ATP with CTP, UTP, and GTP. A radio labelled 13-mer RNA sequence was used as a marker (M) with nt lengths indicated on the left. Source data are provided as a source data file. Template nucleotides (green), incorporated nucleotides (light grey), and 32p ATP nucleotides (yellow) are shown. The experiment was repeated three times with the same result.
