Fig. 6: A minimal stochastic model reveals the impact of cooperativity on the coordination of transcriptional repression.
a Scheme of the Markovian model. b Representation of estimated bursting dynamics for snaMS2 during stable repression in nc14 (Supplementary Movie 1). Permissive ON state durations are depicted in green and inactive OFF states in red and orange, and probabilities of each state shown above. c Best stochastic model fit (yellow) of the RNA production rate parallels the Hill function model fit (black) and the experimentally derived values (teal) over 30 min of minimal stochastic model fitted transcription. Error represents the upper and lower bounds of the 95% confidence interval from all movies. d Comparison of the BCPD-derived repression onset times for the experimentally derived data (teal) and kernel density estimate for simulated data from C (yellow). Median repression onset time is indicated by dashed lines. As the model was not fitted to the experimental distribution, the good agreement suggests its validation. Significance determined by Brown-Mood median test (two-sided). e Distribution of simulated repression onset time for various Hill coefficients for θ constrained to 6.1 <θ < 6.2.
