Fig. 2: Loss of IWS1 decreases Pol II elongation velocity.
From: IWS1 positions downstream DNA to globally stimulate Pol II elongation
a Boxplot showing fold-changes (dIWS1/control) in Pol II occupancy (mNET-seq) after 1 h depletion of IWS1. Representations as in Fig. 1c. p < 2.2 × 10−16. b Heatmap representation of changes in mNET-seq signal in expressed genes (n = 10,147) after depletion of IWS1. Gene regions are aligned at TSS. The black line represents ends of the genes and bins without signal are shown in gray. c As in (b), but for Pol II ChIP-seq signal. d Heatmap representation of changes in Pol II elongation velocity after 1 h depletion of IWS1. Representation as in (b). e Boxplots show mean TT-seq/mNET-seq ratios per gene (n = 10,147). p-values were determined using two-sample two-sided Wilcoxon test, and indicated as follows: n.s = p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Representation as in Fig. 1c. p < 2.2 × 10−16. f Boxplots of intron retention ratios (IR-ratio) per gene in TT-seq. Representation as in e. p < 2.2 × 10−16.
