Fig. 3: IWS1 stimulates Pol II elongation in vitro.
From: IWS1 positions downstream DNA to globally stimulate Pol II elongation
a Schematic representation of the nucleosome-positioning DNA template used for RNA extension assays. RNA primer is shown in red, the template and non-template DNA strands are shown in blue and cyan, respectively. b Denaturing PAGE analysis of RNA extension assays. Fluorescently labeled RNA primer was extended by the activated elongation complex ± IWS1 ± ELOF1. RNA lengths and the main extension products are indicated on the left and right side, respectively. c Quantification of the full-length transcripts from the RNA extension assay shown in b). Data represent mean ± SEM from four independent replicates. p-values were determined using one-way ANOVA followed by Tukey’s multiple comparisons test, and indicated as follows: n.s = p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Statistical significance was observed between three conditions (+/− IWS1 (p = 2.7 × 10−2), +/− both IWS1 and ELOF1 (p = 8.4 × 10−7) and +/− ELOF1 with IWS1 present (p = 4.1 × 10−5). No significance was observed between +/− ELOF1 alone (p = 0.5).
