Fig. 3: Multi-organ proteomic and phosphoproteomic analysis.

a MPXV DNA loads in multiple organs sampled from rhesus macaques necropsied at 10 days post-MPXV infection (dpi). The intensity of blue in the heatmap indicates the level of MPXV DNA load in each organ. The organs were categorized into four classes: lymph nodes, immune tissues, gut, and other tissues. b Study design for the proteomic and phosphoproteomic analysis using eight organs in the MP and MS groups. Created in BioRender. Yang, C. (2025) https://BioRender.com/3zt44aw. c Reliable proteins in eight organs of MP and MS groups. Differentially expressed proteins were calculated using Student’s t test (two-sided, thresholds set at P-adjust < 0.05 and FC > 1.5 or FC > (1/1.5)) in each organ comparing MP to Ctr or MS to Ctr. d Reliable phosphosites in eight organs of the MP and MS groups. Differentially expressed phosphosites were calculated using Student’s t test (two-sided, thresholds set at P-adjust < 0.05 and FC > 1.5 or FC > (1/1.5)) in each organ comparing MP to Ctr or MS to Ctr. e KEGG enrichment results (Fisher’s exact test P < 0.05, two-sided) for unique upregulated or downregulated proteins in each organ comparing the MS to MP group. The red and blue arrows at the bottom of the panel respectively indicate the upregulated and downregulated proteins in the corresponding columns. f Kinase prediction results comparing the MS to the MP group in each organ. Kinases were predicted using GPS software (setting organism at Macaca mulatta and threshold at medium). Kinase activities were predicted using GSEA. The ‘*’ symbol indicates the marked kinase had significantly changed activity (GSEA P < 0.05 and |NES | > 1). Source data are provided as a Source Data file.