Fig. 5: N protein phosphorylation weakens RNA binding affinity. | Nature Communications

Fig. 5: N protein phosphorylation weakens RNA binding affinity.

From: Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states

Fig. 5: N protein phosphorylation weakens RNA binding affinity.

a Quantification of binding affinity between N vs. pN and the viral stem loop 4 RNA (SL4) based on a change in normalized fluorescence polarization (minimum polarization set to 0). Data presented as mean values ± SD, n  =  3 independent trials. b Quantification of binding affinity between N vs. pN and unstructured 30-base polyrA from normalized fluorescence polarization. Data presented as mean values ± SD, n  =  3 independent trials. Source data are provided as a Source Data file.

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