Fig. 3: Recombination events observed in the mating progeny of Ssste50-1 and Ssatg4. | Nature Communications

Fig. 3: Recombination events observed in the mating progeny of Ssste50-1 and Ssatg4.

From: Normal meiosis in the fungus Sclerotinia sclerotiorum despite the irregular distribution of haploid chromosomes between two nuclei

Fig. 3

a Colony morphology of Ssste50-1 and Ssatg4 on PDA plates. The pictures were taken at 14 dpi. b Genetic dissection of mating between Ssste50-1 and Ssatg4. The genotypes of Ssste50-1 and Ssatg4 mutants are denoted as zR and Zr, respectively. At the Ssatg4 locus, R represents the WT allele and r is the mutant Ssatg4 allele. At the Ssste50-1 locus, Z represents the WT allele and z is the mutant Ssste50-1 allele. Following mating, the resulting diploid fusion cell goes through meiosis and gives rise to four types of gametes with equal probability. These gametes produce progeny with three phenotypic classes, segregating in a 1:1:2 ratio (WT-like: Ssatg4-like: Ssste50-1-like) according to the Mendelian law. c A summary table showing the SNP/INDEL polymorphisms detected by NGS and their presence in the genomes of an apothecium and four WT-like colonies derived from ascospores originated from the mating of Ssste50-1 and Ssatg4. The table includes chromosome number (Chrom), gene code, and single nucleotide polymorphism/short insertion or deletion (SNP/INDEL) in red or black, indicating markers derived from Ssste50-1 or Ssatg4, respectively. Two gene codes connected by a dash (-) indicate that the SNP is located in the intergenic region between them. Genes with causal mutations in Ssste50-1 and Ssatg4 are highlighted in bold. The results from NGS analysis are presented as two numbers in each cell, color-coded for clarity. The number before the comma represents the SNP counts of the indicated marker in the reference genome (WT S. sclerotiorum 1980), while the number after the comma represents the SNP counts of the indicated marker in the mutant genome. For all sequenced progeny, cyan indicates markers from Ssatg4, and green indicates markers from Ssste50-1. d Chromosome maps of the selected progeny based on the marker information from c. The chromosome regions containing only Ssatg4 markers are arbitrarily colored in cyan, extending either to the end of the chromosome or to the appearance of an Ssste50-1 marker. Similarly, regions containing only Ssste50-1 markers are arbitrarily colored in green, extending either to the end of the chromosome or to the appearance of an Ssatg4 marker. Chromosome regions with no marker information are shown in white. The maps were drawn using MapChart (v2.32). Note that the chromosome map does not fully represent the true genetic arrangement due to the limitations in the number of available markers.

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