Fig. 6: Microscopic observation of ascospore development in apothecia of the S. sclerotiorum SsH4-mCherry strain. | Nature Communications

Fig. 6: Microscopic observation of ascospore development in apothecia of the S. sclerotiorum SsH4-mCherry strain.

From: Normal meiosis in the fungus Sclerotinia sclerotiorum despite the irregular distribution of haploid chromosomes between two nuclei

Fig. 6

Representative images showing the ascospore formation process, scale bar = 10 μm. a A potential tetrakaryotic cell (or zygote) is pointed out with a white rectangle. b A thin thread (young ascus) containing four gametes. White labels n1 and n2 denote the two nuclei in each cell, while white arrows point to septa stained with Calcofluor-White. c Formation of the mature ascus with eight binucleate haploid ascospores. The apothecial stages used to visualize the different cells are shown below, scale bar = 5 mm. The experiment was independently repeated at least three times with similar results.

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