Fig. 2: Experimental assessment of chronological lifespan.
From: Translational fidelity and longevity are genetically linked
a Overview of the experimental pipeline. The strains were inoculated into YPD medium for 24 hours of cultivation, and then transferred to SC medium for a 15-day aging culture. The relative survival rates of cells on days 2, 4, 6, 9, 12 and 15 were estimated by transferring a small (5 μL) subculture to YPD medium and assessing the time at which the growth of the subcultures exceeds a certain OD threshold. Finally, the chronological lifespan was estimated by the area under the age-survival rate curve. b Our experimental pipeline successfully recapitulated the known lifespan-lengthening effect of carbon restriction. Chronological lifespans of BY in 0.5% (n = 3) and 2% (n = 4) glucose YPD media were individually shown as dots, with their average value and standard deviation respectively indicated by the bars and error bars (one-tailed Student’s t-test P = 2.5 × 10-4). c Estimated lifespans are highly reproducible. The chronological lifespans of segregant strains were assessed in two batches. Seven randomly chosen strains were assayed in both batches, which appears highly consistent. No significant between-batch difference was detected by the Wilcoxon rank-sum test for each strain. Pearson’s Correlation Coefficient R and corresponding P values from two-tailed Pearson’s correlation test are indicated. Each dot represents the average lifespan of three biological replicates, with horizontal and vertical error bars represent standard deviation of three biological replicates in batch 1 and batch 2, respectively. d Overview of the 804 chronological lifespans obtained. The upper panel displays a histogram for the distribution of lifespans averaged across three replications per strain. The lower panel shows each strain individually, with a dot representing the average lifespan and a horizontal error bar depicting the standard deviation across replications. The strains were sorted by increasing average lifespan from bottom to top. Dashed line and error bar respectively represent the average and standard deviation of parental strain lifespans, using colors indicated by the legend.
