Fig. 8: The levels of SLC1A5, HDAC5, and CYP19A1 in the ovaries of DHEA-induced PCOS-like and PAMH mice.

a Schematic diagram of the DHEA-induced PCOS-like model. Created in BioRender. Ye, L. (2025) https://BioRender.com/yb2yu8f. b–d mRNA expression of Slc1a5 (b), Hdac5 (c), and Cyp19a1 (d) in ovaries collected from Control and DHEA-treated mice determined by qPCR (n = 12 for each group). e–h Western blots (e) and densitometric analysis of SLC1A5 (f), HDAC5 (g), and CYP19A1 (h) expression relative to α-Tubulin in the ovaries of Control and DHEA-treated mice (n = 5 for each group). i Schematic diagram of the PAMH model. Created in BioRender. Ye, L. (2025) https://BioRender.com/0wojt74. j Levels of glutamine in the ovaries of F1 female offspring of PBS and AMH-treated (n = 5 for each group) dams. k–m mRNA expression of Slc1a5 (k) Hdac5 (l) and Cyp19a1(m) in the ovaries of F1 female offspring of PBS and AMH-treated dams determined by qPCR (n = 15 for each group). n–q Western blots (n) and densitometric analysis of SLC1A5 (o) HDAC5 (p) and CYP19A1 (q) expression relative to β-Actin in the ovaries of PBS and AMH-treated mice (n = 5 for each group). For statistical analysis, Mann-Whitney tests were performed in (b, c, m); and Student’s t tests were performed in (d, f–h, j–l, o–q). All tests were two-sided. The biological replicates are used for statistical analysis in (b–h, j–q). Source data are provided as a Source Data file.