Fig. 7: Dal81 and Stp2 coordinate to activate transcription of alkalinization-responsive genes.

A Genome-wide distribution of candidate Dal81 or Stp2 binding regions from ChIP-seq assays in untagged wild-type, Stp2-HA, and Dal81-Myc strains grown in alkalinization-inducing medium (YNB + 1% CAA, initial pH 4.5). B De novo motif analysis using HOMER identified consensus binding motifs for Dal81 and Stp2 at their binding sites. C Venn diagram showing overlap between Dal81 and Stp2 binding sites. D GO enrichment analysis of common targets genes shared by Dal81 and Stp2. E ChIP-qPCR validation for Dal81 binding to PUT1, GDH2, and PCK1 in Dal81-Myc or Dal81-Myc (stp2Δ/Δ) strains, shown as fold enrichment of the signal from immunoprecipitation relative to the input DNA. WT served as the negative control. F ChIP-qPCR validation for Stp2 binding to PUT1, GDH2 and PCK1 in Stp2-HA or Stp2-HA (dal81Δ/Δ) strains. G ChIP-qPCR validation for Stp2 binding to PUT1, GDH2 and PCK1 in Dal81-Myc or Dal81m21-Myc strains. H–M RT- qPCR analysis of PUT1, GDH2 and PCK1 mRNA abundance in different strains under alkalinization conditions. Values for each gene were normalized against ACT1. Data are means ± SD of three biological replicates. Statistical significance via unpaired two-tailed Student’s t-test (E, F, H–J) and one-way ANOVA with Tukey’s multiple comparisons test (K–M). * P < 0.05, ** P < 0.01, ***P < 0.001, **** P < 0. 0001; ns: not significant. Source data for (E–M) are provided as a Source Data file.