Fig. 7: Treatment efficacy of NMSs against IRI rats at 6 h post-surgery. | Nature Communications

Fig. 7: Treatment efficacy of NMSs against IRI rats at 6 h post-surgery.

From: Biosilica nanoparticulate scavengers for the therapy of hepatic ischemia–reperfusion injury in preclinical models

Fig. 7: Treatment efficacy of NMSs against IRI rats at 6 h post-surgery.

a. Treatment protocols on the rat IRI model harvested at 6 h post-surgery. HE staining images (b), ischemic areas, SUZUKI score, and serum biochemical indexes (c), cfDNA level (d), and cytokine levels (e) of IRI rats after 60 min of ischemia and 6 h of reperfusion. n = 3 (HE staining) or 4 biologically independent animals. f DHE staining images of the liver tissues of IRI rats; blue: cell nucleus, red: ROS. g CD31 staining images of the liver tissues of IRI rats; blue: cell nucleus, green: endothelial. h Caspase-3 staining images of the liver tissues of IRI rats; blue: cell nucleus, red: cell apoptosis. i Tunel staining images of the liver tissues of IRI rats; blue: cell nucleus, green: apoptotic cells. j F4/80 (as a M1 macrophages marker, white), CD86 (as a M1 macrophages marker, green) and CD206 (as a M2 macrophages marker, purple) staining images of the liver tissues of IRI rats to indicate macrophages. k CD11b (as a myeloid cells marker, white), Ly6G (as a neutrophil marker, green) and MPO (as a neutrophil marker, purple) staining images on liver tissues of IRI rats to indicate neutrophil. n = 3 biologically independent animals. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001 by one-way ANOVA. Source data are provided as a Source Data file.

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