Fig. 1: Serotonin (5-HT)-mediated presynaptic depression at the anterior cingulate cortex (ACC) to claustrum (CL) synapse. | Nature Communications

Fig. 1: Serotonin (5-HT)-mediated presynaptic depression at the anterior cingulate cortex (ACC) to claustrum (CL) synapse.

From: Serotonin and psilocybin activate 5-HT1B receptors to suppress cortical signaling through the claustrum

Fig. 1

A Diagram detailing the experimental approach. B Representative image of the ACC injection site and terminal expression in claustrum. C Higher magnification of image in panel B depicting ACC terminal expression in the claustrum; Cortex (CTX) and striatum (STR) are also labeled. D Diagram of whole-cell patch recordings with optical stimulation of ACC terminals, corresponding to E–G. E 10 min of 10 μM 5-HT induced a depression of optically evoked post synaptic potential (oEPSC) amplitude. F Top, normalized oEPSC amplitude is significantly lower during the last five minutes of recording in neurons exposed to 10 min of 10 μM 5-HT (n = 13, from 8 animals) compared to a no-drug control (n = 6 neurons, from 4 animals) (two-tailed Student’s t-test, t16 = 2.53, p = 0.022). Bottom, 5-HT (10 min, 10 μM) significantly increased paired-pulse ratio (PPR) (two-tailed paired Student’s t-test, t12 = 3.18, p = 0.008, n = 13 neurons from 8 animals). G Representative traces of paired-pulse oEPSC events during baseline and the last five minutes of recording for both 5-HT-exposed neurons (blue) and no-drug control neurons (black). H Diagram of whole-cell patch recordings with optical stimulation of ACC terminals, corresponding to I–K. I The 5-HT- (10 min, 10 μM) mediated depression of oEPSC amplitude persisted in the presence of TTX and 4-AP. J The oEPSC amplitude during the last 5 min of recording is not significantly different between 5-HT/TTX/4-AP (n = 10, from 4 animals) or 5-HT alone (n = 6, from 4 animals) conditions (two-tailed Student’s t-test, t14 = 0.017, p = 0.99). K Representative traces of paired-pulse oEPSC events during baseline and last five minutes of recording for both TTX/4-AP/5-HT (light blue) and 5-HT alone (dark blue) conditions. Scale bars are 1 mm for B, 0.3 mm for C, 25 ms (horizontal) by 200 pA (vertical) for G and K. All error bars represent standard error of the mean (SEM). Source data are provided as a Source Data file.

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