Fig. 1: The compositional heterogeneity in tubulin encapsulation by closed TRiC chamber.
From: Visualizing dynamic tubulin folding in chaperonin TRiC from nonnative nucleus to final native state
Color coding for A–E: TRiC in dark gray; undefined density in orange; N domain in cyan; C domain in green; Core helix domain in blue; Middle domain in red. A 529,181 TRiC particles display six types of compositional heterogeneity within their rings, including the apo state, occupied by undefined density, tubulin folding states I, II, III, IV. B The diagram shows that each of the two rings in a TRiC can possibly be one of six scenarios in (A). C The histogram shows the statistical distribution of TRiC particles with compositional heterogeneity from both rings. * The particle set for Fig. 2. Δ The particle set for Fig. 3. ◊ The particle set for Fig. 4. Color coding: Apo TRiC ring in gray; rings with undefined density in orange, state I in cyan, state II in green, state III in blue and state IV in red, respectively. Source data are provided as a Source Data file. D Representative reconstruction of TRiC with synchronous folding between two rings (left) and representative reconstruction of TRiC with the nonsynchronous folding between two rings (right). E The reconstructions of TRiC when one ring is occupied by undefined density and the opposite ring is either in the apo state or occupied by a tubulin folding intermediate I.
