Fig. 3: Performance of OK-MLIR in living cells.
From: Dual-key cooperatively activated DNA regulator for controlling mitochondria-lysosome interactions
a Confocal fluorescence images of HeLa cells transfected without (Blank), and with 180 nM CD63APT-TPP, OK-MLIR and OK-MLIR in presence of 10-min UV irradiation. Scale bar: 30 µm. MitoRed, LysoBlue and FAM were excited with 579, 373 and 494 nm lasers, respectively. b Pearson Correlation analysis of (a) by investigating the fluorescence signals of MitoRed and LysoBlue. Shown are mean ± SEM from ten individual cells. (Blank vs CD63APT-TPP, P = 5.45 × 10−16 and OK-MLIR vs OK-MLIR (UV), P = 3.00 × 10−11, ****P < 0.0001, two-tailed Student’s t test). c Super-resolution images of HeLa cells transfected without (Blank), and with 180 nM CD63APT-TPP, OK-MLIR and OK-MLIR in presence of 10-min UV irradiation. MitoTracker Green and LysoTracker were excited with 490 and 580 nm lasers, respectively. Scale bar: 10 µm. d TEM images of HeLa cells (Blank) and HeLa cells treated with 180 nM CD63APT-TPP, OK-MLIR and OK-MLIR in presence of 10-min UV irradiation. Scale bar: 5 µm. e Confocal images of HeLa cells (Blank) and HeLa cells treated with 180 nM CD63APT-TPP, OK-MLIR, and OK-MLIR in presence of 10-min UV irradiation. MtphagyDye, LysoDye and MitoBright DeepRed were used for staining mitochondrial autophagy, lysosomes, and mitochondria, respectively. Scale bar: 50 µm. f Confocal immunofluorescence staining images of HeLa cells treated without (Blank) and with 180 nM CD63APT-TPP, OK-MLIR and OK-MLIR in presence of 10-min UV irradiation., then labeled with COXIV antibody (green) and DAPI (blue). Scale bar: 100 µm. g Corresponding green fluorescence intensities were extracted from (f) for quantitative analysis. Shown are mean ± SEM from ten individual cells. (Blank vs CD63APT-TPP, P = 6.70 × 10−9 and OK-MLIR vs OK-MLIR (UV), P = 7.31 × 10−7, ****P < 0.0001, two-tailed Student’s t test). h Western blot map of TOMM20 protein in HeLa cells treated without (Lane 1) and with CD63APT-TPP (2), OK-MLIR (3), and OK-MLIR in presence of 10-min UV irradiation (4). Image representation of 3 experiments. Source data are provided as a Source Data file.
