Fig. 2: Preimplantation high-fat diet (HFD) exposure altered sncRNA expression in OF and UF.

a Schematic overview of the experimental workflow. b–c Comparison of tsRNAs (b) and rsRNAs (c) expression in OF and UF under normal diet (ND) and HFD. Box plots display the median (center line), 25th and 75th percentiles (box bounds), and whiskers extending to the minimum and maximum values within 1.5× the interquartile range (IQR). Individual data points represent each sample and are overlaid as dots on the box plots, two-tailed Student’s t-tests, n = 3 biologically independent experiments, each using pooled samples from 4 mice. d Relative ratio of tsRNAs to rsRNAs in different OF and UF, two-tailed Student’s t-tests, n = 3 biologically independent experiments, each using pooled samples from 4 mice. e–f Changes of mitochondrial and genomic tsRNAs (e) and rsRNAs (f), mt-tsRNA: mitochondrial tsRNA, cyto-tsRNA: genomic tsRNA, mt-rsRNA: mitochondrial rsRNA, cyto-rsRNA: genomic rsRNAs, two-tailed Student’s t-tests, n = 3 biologically independent experiments, each using pooled samples from 4 mice. g Changes of amino acid corresponds tRNA origins. h Changes of rsRNAs origin loci, RPM: Reads Per Million mapped reads. i Changes of UF RNA modifications in response to HFD exposure. The red font indicates upregulated RNA modifications, blue font indicates downregulated RNA modifications. n = 3 biologically independent experiments, each using pooled samples from 4 mice. The data represent means ± s.e.m. Two-tailed multiple t-tests, ^*P ≤ 0.05, ^**P ≤ 0.01. Statistical source data, precise P value and unprocessed blots are provided as in the Source Data file.