Fig. 5: Early embryo changes by transfecting changed sncRNAs. | Nature Communications

Fig. 5: Early embryo changes by transfecting changed sncRNAs.

From: Maternal diet-induced alterations in uterine fluid sncRNAs compromise preimplantation embryo development and offspring metabolic health

Fig. 5: Early embryo changes by transfecting changed sncRNAs.The alternative text for this image may have been generated using AI.

a Representative images of blastocysts after transferring synthesized FAM-labeled sncRNAs (green). Scale bar = 10 μm, n = 3 biologically independent experiments. b PCA plot of blastocysts transfected with empty liposome (control) or sncRNA pool. c Expression heatmaps of genes in control and sncRNA pool transfected blastocysts. d Pathway changes after transfection, two-tailed Student’s t-tests, n = 3 biologically independent experiments. e Specifically changed genes. f–g Predicted interactions between transfected tsRNAs (f) and rsRNAs (g) with changed genes. h–j Significant correlations between sncRNAs in UF and altered genes within blastocysts, tsRNA-Leu-AAG and Hs6st1 (h), mt-tsRNA-Trp-TCA and Ctdspl (i), and rsRNA-18S and B3gnt7 (j), were detected by both correlation patterns analysis and individual sncRNAs transfection in NIH/3T3 cells, n = 3 biologically independent experiments. Two-tailed Student’s t-tests, and the data represent means ± s.e.m. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Statistical source data and precise P value are provided as in the Source Data file.

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