Fig. 5: Role of blaOXA-48 in pOXA-48 fitness effects.
From: Dissecting pOXA-48 fitness effects in clinical Enterobacterales using plasmid-wide CRISPRi screens
The blaOXA-48 gene is responsible for pOXA-48-associated fitness costs. A Two-sided Pearson correlation between the relative Area Under the growth Curve (AUC) of pOXA-48-carrying strains compared to their pOXA-48-free counterparts (shown in Fig. 1D) and the gene score (log2 fold change) associated with silencing blaOXA-48 in the CRISPRi screen. Lines indicate the best fit for the correlation of each species. Shadowed areas indicate the 95% CI. Each colour represents a species. B Plasmid cost associated with carrying pOXA-48 and pOXA-48∆blaOXA-48 in different clinical strains of Enterobacterales measured as relative AUC of pOXA-48-carrying compared to pOXA-48-free clones. Colours of strain names in the x-axis are again indicative of species: in pink E. coli, in blue K. pneumoniae, and in black C. freundii. Asterisks indicate significant cost/benefit of either pOXA-48 or pOXA-48∆blaOXA-48 in each of the strains (two-sided paired t-tests against each relative plasmid-free strain after Bonferroni correction; n = 8, except for KPN11, KPN10, KPN18, K163, EC22, C288 carrying pOXA-48, in which n = 16; p < 0.05; error bars correspond to Standard Deviation).
