Fig. 2: 15N spin relaxation of Dab2320–495 upon interaction with the EH domains.
15N R1ρ spin relaxation of Dab2320–495 (100 µM) in the absence and presence of (A) EH1 or EH3 and (B) increasing concentrations of EH2. The experiments were recorded at a 1H frequency of 600 MHz. The relaxation rates in (A) and (B) were derived from a fit of peak intensities against the relaxation delay. Errors of the fitted rates were derived from the experimental uncertainty. C Zoom into the peak corresponding to V399 from Dab2320–495 1H-15N HSQC spectra alone and in the presence of increasing concentrations of EH1 (left) and EH2 (right). D Zoom into the peak corresponding to L486 from Dab2320–495 1H-15N HSQC spectra alone and in the presence of increasing concentrations of EH1 (left) and EH2 (right). Color legends are displayed in the respective panels. E ΔR2, eff calculated from effective R2 values (R2, eff) of relaxation dispersion experiments at a CPMG frequency of 31 Hz subtracted with R2, eff at a CPMG frequency of 1000 Hz recorded on a sample of 100 µM Dab2320–495 in the presence of 10% EH2 at a 1H frequency of 600 MHz and 900 MHz. Error bars, propagated from the errors of R2, eff at 31 Hz and 1000 Hz determined from repeat measurements and with a minimum error of 0.5 (see Supplementary Fig. 7B for the corresponding decay curves), are shown for values that are significantly larger than 0 .
