Fig. 4: Correlation analysis of mRNAs and proteins across pyramidal neuron compartments.
From: An integrated transcriptomic and proteomic map of the mouse hippocampus at synaptic resolution
a Pearson’s correlation of mean abundances of mRNAs (log2 DESeq2 normalized counts) and their respective protein (log2 intensity-based absolute quantification, iBAQ) across CA1 strata and within each stratum. b Selected examples showing strong positive (≥0.9) and negative (≤ –0.9) Pearson’s correlation in their mean abundance changes across strata. Abundances for individual replicates are shown as translucent lines, while solid lines represent mean values with error bars indicating ± SD (n = 7). c Selected GO terms overrepresented by all transcripts and proteins showing a strong positive or negative correlation in their mean abundance changes across strata. Significance was determined by a one-sided hypergeometric test and -log10 FDR values are indicated on the y-axis. d Protein half-lives of strongly positively and negatively correlated mRNA-protein pairs. A total of 2946 genes were mapped to neuronal protein half-life data from Dörrbaum et al.118. Boxes indicate median (middle line) and interquartile ranges (IQR), with whiskers at 1.5 × IQR. Black translucent dots indicate outlier data points that fall beyond y-axis limits. Significance is indicated by asterisks (**p < 0.01, ****p < 0.0001) and was determined by Kruskal–Wallis test (χ²(2) = 57.8, p = 2.84 × 10−13, df = 2, N = 3692) followed by a post hoc Dunn’s test with Bonferroni correction: all vs. positive (Z = −3.12, p = 0.00181, p adjusted = 0.00542), all vs. negative (Z = 6.54, p = 6.24 × 10−11, p adjusted = 1.87 × 10−10), and positive vs. negative (Z = 7.33, p = 2.29 × 10−13, p adjusted = 6.87 × 10−13). Source data are provided as a source data file.
