Fig. 4: VCPIP1 catalytic activity and interaction with VCP contribute to Golgi assembly.

a Representative western blot, blotting for VCPIP1, demonstrating successful transient transfection of VCPIP1 constructs in A2058 VCPIP1 KO cells. b Representative fluorescent images of A2058 WT and KO cells in which various VCPIP1 constructs were transiently transfected into the cells. Cells were stained with DAPI (first row) and GM130 antibody (second row). Both channels were merged as depicted in the third row. Scale bar is 10 µm (depicted in white). Images, biological triplicates each with technical duplicates, were taken on different days using the same imaging set-up, which may result in differences in apparent brightness. c Representative freeform mask (yellow) drawn in ImageJ Fiji around Golgi signal from one cell from a single merged image from WT and KO conditions are shown. d Quantification of area of Golgi per cell from various conditions are represented in a GraphPad Prism column chart. The data represented as mean ± SD from three biological replicates each with technical duplicates The P values were calculated using one-way ANOVA between KO and all other conditions using GraphPad Prism software (p < 0.0001).