Fig. 4: MOS MGs block AIEC adhesion to intestinal epithelial cells by binding with FimH.
From: Fishing out AIEC with FimH capturing microgels for inflammatory bowel disease treatment
A–C The purified FimH protein was subjected to SPR experiments to detect its molecular interaction with mannose (A), MOS (B), and glucose (C). D Confocal images of MOS MGs (I) and MGs (II) co-cultured with AIEC, as well as confocal images of MOS MGs co-cultured with ΔfimH (III). E Competitive adhesion assay of AIEC between MOS MGs and Caco-2 cells, visualized by colony-forming unit (CFU) plating (n = 3 biological replicates). F Confocal imaging of competitive adhesion of AIEC between MOS MGs and Caco-2 cells. Red: AIEC; blue: DAPI-stained nuclei; green: Calcein-AM-stained cells; white arrows indicate AIEC. G–I Colonization levels of WT in feces (G), colon (H), and ileum (I) at 48 hours post-treatment with MOS, MGs, MOS MGs, and M4284. (n = 6). Significance was determined by one-way ANOVA (E) or two-sided Mann‒Whitney U test (G–I) and indicated as the P-value. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; n.s. no significant difference. Data are presented as mean ± s.d. (E). Source data are provided as a Source Data file.
