Fig. 9: DNA end resection factors bound and function at early M phase following replication stress. | Nature Communications

Fig. 9: DNA end resection factors bound and function at early M phase following replication stress.

From: DNA double-strand break end resection factors and WRN facilitate mitotic DNA synthesis in human cells

Fig. 9

a Experimental workflow for analyzing RPA foci following APH treatment in prometaphase cells coupled with short inhibition to MRE11 (with PFM39), DNA2 (with C5) or WRN (with HRO761) at G2/M boundary. Representative immunofluorescence images (b) and quantification (c) of RPA foci (red). DNA was stained with DAPI (blue). Scale bars, 10 μm. n = 102: over 30 cells were analyzed in each condition in each replicate. In total, 102 cells were analyzed for each condition. d Experimental workflow for analyzing WRN’s localization at loci with replication stress marked by FANCD2 in G2 and early M phase with or without the treatment of APH. Representative immunofluorescence images (e) and quantification of foci (f) that have WRN (red) and FANCD2 (green) co-localization in the nucleus. DNA was stained with DAPI (blue). Scale bars, 10 μm. In each quantification, data are presented as mean ± s.e.m. of at least 3 independent experiments. n = 105: over 30 cells were analyzed in each condition in each replicate. In total, 105 cells were analyzed for each condition. Statistical p values were calculated with two-tailed non-parametric Mann–Whitney tests and indicated in quantification graphs. Source data are provided as a Source Data file.

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