Fig. 5: Developmental polyadenylation regulation equally affects cortical layers more than the white matter. | Nature Communications

Fig. 5: Developmental polyadenylation regulation equally affects cortical layers more than the white matter.

From: A spatial long-read approach at near-single-cell resolution reveals developmental regulation of splicing and polyadenylation sites in distinct cortical layers and cell types

Fig. 5: Developmental polyadenylation regulation equally affects cortical layers more than the white matter.The alternative text for this image may have been generated using AI.

a Percent significant genes with differential polyA sites. Cortex percent significant: 24.67%, WM percent significant: 3.70%. b PolyA deltaPI density in cortex and WM. c Downsampling experiments of percent significant genes with alternative PolyA sites where age groups (n = 4 Child, n = 4 Y.A.) are combined and compared. Sampling considered individuals equally and selected 20 reads and 50 genes randomly. Genes were resampled 100× and calculated percent significant per iteration (“Methods”, n = 100). d ScisorWiz48 example of alternative poly(A) site in the VBP1 gene. e Average length of last exons per tested genes with |deltaPI| < 0.2 compared across regions and age groups. Age groups combine 4 samples each. Child WM: n = 595; Y.A. WM: n = 643; Child Cortex: n = 2350; Y.A. Cortex: n = 2250. f Average length of last exons per genes with |deltaPI| > 0.2 compared across regions age groups. Age groups combine 4 samples each. Child WM: n = 152; Y.A. WM: n = 161; Child Cortex: n = 478; Y.A. Cortex: n = 470. g Average length of UTR per genes with |deltaPI| > 0.2 compared across regions age groups. Age groups combine 4 samples each. Child WM: n = 386; Y.A. WM: n = 392; Child Cortex: n = 552; Y.A. Cortex: n = 551. h Overview of all cortical overlapping genes that were tested in both Poly(A) and exon tests (n = 2096). Genes were classified as only having |deltPI| > 0.2, only having a |deltaPSI| > 0.2, or both. **** = P ≤ 0.0001, *** = P ≤ 0.001, ** = P ≤ 0.01, * = P ≤ 0.01. Boxplot center line is at the median, upper bound at the 75th percentile, lower bound at the 25th percentile with whiskers at 1.5 × IQR. WM is colored in blue and Cortex is colored in red. A two-sided Wilcoxon rank-sum test was applied to all the comparisons shown in (c, e–g).

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