Fig. 3: Contextual epistasis shapes TL evolution.
From: Widespread epistasis shapes RNA polymerase II active site function and evolution
a Schematic for the TL evolutionary haplotypes library. We selected 662 TL haplotypes representing TL alleles from bacterial, archaeal, and the three conserved eukaryotic msRNAPs. These TL alleles were transformed into yeast and were phenotyped under selective conditions. b Fitness of evolutionarily observed TL haplotypes in the yeast Pol II background. The Pol II WT TL fitness (0) is labeled as a dotted line. Kruskal–Wallis test was performed for comparison, and significant levels (Bacteria vs. Archaea, P > 0.9999. Bacteria vs. Pol I, P = 0.0027. Bacteria vs. Pol III, P = 0.0003. Bacteria vs. Pol II, P < 0.0001) were labeled.
