Fig. 3: Neutrophil, monocyte, and dendritic cell dynamics in blood and BAL of HTLV-1A and HTLV-1A/C_oI-L infected macaques.

Beeswarm boxplots depict cell population frequencies of a plasmacytoid dendritic cells (pDCs) and myeloid dendritic cells (mDCs) and their subsets expressing IL-8, IL-10, or TNF-α markers. b Total monocytes, with the three monocyte populations (CD14⁺CD16⁻ classical, CL; CD14⁻CD16⁺ non-classical, NC; and CD14⁺CD16⁺ intermediate, IN), and their subsets expressing IL-8, IL-10, both CD162 and IL-10, or TNF-α markers. c Neutrophils and their subsets expressing IL-8, IL-10, both CD162 and IL-10, TNF-α, CD11b, CD64, or MPO markers in blood (left) and BAL (right). The x-axis represents cell frequency. Only cell populations with p < 0.05 by Mann–Whitney test between the two triple-depleted groups at weeks (Wk) 5, 12, or 21 are shown and sorted by timepoint (top to bottom, separated by thick black lines), then by direction of the HTLV-1A/C_oI-L vs HTLV-1A difference. P value ranges are indicated by asterisks: *p < 0.05, **p < 0.01, ***p < 0.001. Alluvials connect the same cell populations in the blood and BAL over time. Bulk population and subset frequencies are displayed above and below the dotted line, respectively. For bulk populations, alluvials are colored according to their pattern of significance with consistently HTLV-1A/C_oI-L > HTLV-1A (dark green), or HTLV-1A/C_oI-L < HTLV-1A (light green) at all significant timepoints/compartments. Purple shading indicates HTLV-1A/C_oI-L greater than HTLV-1A, and HTLV-1A/C_oI-L less than HTLV-1A at different timepoints/compartments. For subsets, alluvials are colored by marker cytokines as indicated. Alternative visualizations of this data can be found in Supplementary Figs. 6 and 7. While each dark and light green symbol corresponds to an animal from the triple-depleted groups inoculated with HTLV-1A/C_oI-L or HTLV-1A, respectively, closed and open symbols correspond to animals belonging either Study 1 or Study 2, respectively. For box and whiskers plots, the center is the median (thick gray bar), bounds of the boxplot are the 25th and 75th percentiles, and whiskers extend from these bounds to the maximum and minimum value no further than 1.5 times the distance between the first and third quartiles. Source data are provided as a Source data file.