Table 1 Mapping and comparison of lysine solvent accessibilities to Cryo-EM structures
From: In vivo conformational space and defects of misfolded CFTR variants by covalent protein painting
Position | Sequence | Mean surface accessibility (%) | Wt Cryo-EM structure | Wt Cryo-EM structure, ATP bound, phosphorylated | |
|---|---|---|---|---|---|
wt | ∆F508 | ||||
K52 | QIPSVDSADNLSEKL | nd | 92.6 | accessible (93.75 Å2) | accessible (13.39 Å2) |
K95 | LGEVTKAVQPL | 96.1 | 77.0 | partially accessible (3.51 Å2) | accessible (35.78 Å2) |
K273 | VITSEMIENIQSVKAY | 61.8 | 95.0 | inaccessible (0.48 Å2) | partially accessible (6.92 Å2) |
K283 | CWEEAMEKMIENLRQTEL | 98.7 | 98.9 | accessible (53.98 Å2) | accessible (39.15 Å2) |
K370 | DSLGAINKIQDF | 99.8 | 99.0 | accessible (41.45 Å2) | accessible (29.92 Å2) |
K370/K377 | DSGAINKIQDFLQKQEY | 99.8 | 98.9 | accessible (41.45 Å2, 39.11 Å2) | accessible (29.92 Å2, 36.11 Å2) |
K411 | not present in CryoEM | ||||
K420 | not present in CryoEM | ||||
K442 | SLLGTPVLKDINF | 99.2 | 95.7 | accessible (39.53 Å2) | accessible (47.84 Å2) |
K464 | AVAGSTGAGKTSLL | 99.7 | 93.0 | accessible (9.59 Å2) | partially accessible (4.36 Å2) |
K536 | AEKDNIVLGEGGITL | 98.8 | 94.7 | accessible (51.94 Å2) | accessible (26.99 Å2) |
K584 | GYLDVLTEKEIF | nd | 91.9 | accessible (8.91 Å2) | accessible (18.03 Å2) |
K1060 | ESEGRSPIFTHLVTSLKGLW | 99.0 | 98.5 | accessible (49.05 Å2) | partially accessible (3.15 Å2) |
K1080 | FETLFHKAL | 99.9 | 99.7 | accessible (59.33 Å2) | accessible (35.59 Å2) |
K1165 | KFIDMPTEGKPT | 99.5 | 96.6 | accessible (19.66 Å2) | accessible (54.57 Å2) |
K1218 | TAKYTEGGNAILENISF | nd | 89.3 | accessible (41.39 Å2) | accessible (30.56 Å2) |
K1292 | GVIPQKVF | nd | 94.4 | accessible (48.20 Å2) | partially accessible (3.01 Å2) |
K1302 | RKNLDPYEQWSDQEIW | 98.4 | 97.9 | accessible (26.64 Å2) | accessible (13.86 Å2) |
K1317 | KVADEVGLRSVIEQFPGKLDF | 99.7 | 99.5 | accessible (37.29 Å2) | accessible (53.09 Å2) |
K1334 | RSVIEQFPGKLDF | 99.9 | nd | accessible (56.92 Å2) | accessible (49.60 Å2) |
K1420 | LVIEENKVRQY | 99.3 | 94.8 | accessible (51.71 Å2) | accessible (51.65 Å2) |
K1429 | DSIQKLL | 99.9 | 99.5 | accessible (57.27 Å2) | accessible (33.64 Å2) |
