Fig. 7: A working model of PFDN-RV VP3 interactions.
From: Prefoldin complex promotes interferon-stimulated gene expression and is inhibited by rotavirus VP3
PFDN interacts with UBA3 and ensures its correct folding. UBA3 is essential for ISG production. In RV-infected cells, VP3 protein competes with UBA3 for interaction with PFDN. This competition results in the inhibition of ISG expression, thereby benefiting RV replication. In PFDN deficient cells, UBA3 levels are decreased, leading to inhibited ISG production and increased virus replication. RV with VP3 critical mutations cannot bind PFDN and inhibit UBA3 and thus have reduced infectivity. Image was created using Adobe Illustrator.
