Fig. 4: Quantitative analysis of HLA-presented uORF/uoORF-derived peptides in mitotically arrested U-2 OS cells.

a Volcano plot illustrating the label-free quantification of the immunopeptidome in Taxol-treated versus DMSO-treated U-2 OS cells, with genes expressing uORF/uoORF-derived peptides highlighted. Peptides with a log2 fold change >0.5 and adjusted P value <0.05 are marked in red, while those with a log2 fold change <−0.5 and adjusted P value <0.05 are marked in blue. Statistical analysis was performed using an empirical Bayes moderated t-test with two-sided P values. Data from biologically independent experiments (n = 3). b Name of the gene expressing the uORF/uoORF-derived peptide, log2 fold change (FC) of peptide abundance, and peptide sequence of mitotic arrest-induced peptides in U-2 OS cells. Data from biologically independent experiments (n = 3). c Read distribution of representative uORFs/uoORFs with increased expression in mitotically arrested U-2 OS cells. Ribo-Seq (upper panels) and RNA-Seq (lower panels) reads are displayed for the 5’ UTR and the start of the coding sequence (CDS).