Fig. 4: Molecular features of striatal astrocytes from aged mice.
From: Aging in mice alters regionally enriched striatal astrocytes
a Top aging astrocyte markers were assessed using MAST differential expression, and the top 25 up and 25 downregulated transcripts are shown in the heatmap (see also Supplementary Data 5). Dorsal enriched genes are marked with an asterisk (*) and ventral enriched genes are marked with a hashtag (#). b Representative image of MERFISH RNA counts shows age increases in Gfap in the dorsal striatum. c Representative images of the dorsal striatum are shown for young and aged mice. GFAP coverage per 500 μm2 in the dorsal striatum; across young and aged mice (n = 4–5 mice; 2-way RM ANOVA with Bonferroni post hoc (*p = 0.00032 (aged dorsal compared ventral) and *p = 0.00029 (aged dorsal compared to young dorsal), data are presented as mean values ± SEM). d Representative image of MERFISH RNA counts shows S100b expression in the dorsal striatum. e Quantification of S100β + cells per 500 μm2, in the dorsal, medial, and ventral striatum; across young and aged mice (n = 4–5 mice; 2-way RM ANOVA with Bonferroni post hoc *p = 0.036, data are presented as mean values ± SEM). f IPA analysis was performed on age-induced DEGs in striatal astrocytes, and each black bar indicates the number of genes per pathway, circle size indicates the −log(p value) (right-tailed Fisher’s Exact Test), and circle color indicates the activation score number. g Aging gene score is calculated by the average expression levels of the top 20 differentially expressed genes in age on a single-cell level, subtracted by the aggregated expression of control feature sets. Each dot represents an astrocyte, and the color of the dot is the relative change in the aging score. h Heatmaps of the top 10 shared genes between our aging and A1 astrocyte subtype genes (Log2FC > 0.01) with mouse aging (Log2FC > 0.01), human striatal astrocyte aging, human striatal astrocyte Huntington’s Disease, and human Parkinson’s Disease using the DEG (see data availability and Supplementary Data 5). Venn diagrams visualize the total overlap across each gene set. i UpSet plot of the overlap of murine aging striatal data and the past studies.
