Fig. 4: Modulation of the pyroptosis-induced IME enhances osteoblast differentiation. | Nature Communications

Fig. 4: Modulation of the pyroptosis-induced IME enhances osteoblast differentiation.

From: Pyroptosis-responsive microspheres modulate the inflammatory microenvironment to retard osteoporosis in female mice

Fig. 4

A TEM image of BMSCs after different treatments. Scale bars: 5 μm. B The expression of pyroptosis-related proteins in BMSCs was detected by Western blotting. C The expression of GSDMD in BMSCs was detected via CLSM. Scale bars: 50 μm. D Quantitative analysis of the expression of GSDMD in BMSCs was detected by CLSM (n = 5 independent experiments). E Changes in the levels of inflammatory factors within cell supernatants were detected via ELISA (n = 5 independent experiments). F ALP staining of BMSCs after 7 days and 14 days. Scale bars: 200 μm. G ARS staining of BMSCs after 14 days and 21 days. Scale bars: 200 μm. H The expression of osteogenic differentiation-related proteins in BMSCs was detected by Western blotting. I The GelMa/Mg/DMF MSs promoted osteogenic differentiation by targeting the N-GSDMD to inhibit pyroptosis in BMSCs and thereby orchestrated the IME. (created with BioRender.com) The data are presented as the means ± SD. Statistical significance was calculated by one-way ANOVA with Tukey’s post hoc test. Source data are provided as a Source Data file.

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