Fig. 5: The molecular myelin framework is altered in Gtf2i-KO mice, with increased expression levels of key myelin factors, along different developmental stages.
A Heatmap showing the relative abundance of selected myelin proteins in Gtf2i-KO compared to control myelin derived from the hemisphere of P30 mice. Data represent n = 3 mice per genotype analyzed as four technical replicates per mouse. Note that the relative abundance of several proteins was significantly increased (Mog, Mbp, Padi2) or decreased (Gpr37, Epb41l3, Cfl1) in Gtf2i-KO mice myelin. The abundance of Cnp was reduced in Gtf2i-KO myelin reflecting that the Cre driver line possesses only one Cnp allele. Exact q-values are given in Supplementary Data 1 and were calculated by moderated t-statistics and corrected for multiple comparisons using the Bioconductor R packages ‘limma’ and ‘q-value’, respectively. * q < 0.05, *** q < 0.001 (B) Mbp protein expression level is significantly elevated in the myelin fraction derived from the cortices of Gtf2i-KO mice, as compared to controls (n = 13 control, n = 12 Gtf2i-KO, P = 0.048). C Mbp mRNA expression level in the myelin fraction derived from the cortices of P30 mice is significantly elevated in Gtf2i-KO mice, as compared to controls (n = 11 control, n = 8 Gtf2i-KO, P = 0.0067). D–F Quantitative PCR (qPCR), whole cortex mRNA, P30 mice. D Sox10 (n = 12 control, n = 12 Gtf2i-KO, P = 0.0003) E Mbp (n = 14 control, n = 16 Gtf2i-KO, two-sided Welch’s t-test, P = 0.0006), and F Mog (n = 12 control, n = 13 Gtf2i-KO, P = 0.0071) mRNA expression levels are significantly elevated in Gtf2i-KO mice, compared to controls. G, H Western blot assay on myelin fraction derived from the cortices of P90 mice. G Mbp (n = 12 control, n = 10 Gtf2i-KO, P = 0.0247) and H Mog (n = 12 control, n = 11 Gtf2i-KO, P = 0.008) protein expression levels are significantly elevated in Gtf2i-KO mice, compared to controls. I–K qPCR, whole cortex mRNA, P90 mice. I Sox10 (n = 13 control, n = 14 Gtf2i-KO, P = 0.0269), J Mbp (n = 14 control, n = 15 Gtf2i-KO, P = 0.006), and K Mog (n = 13 control, n = 15 Gtf2i-KO, P = 8.3*10−5) mRNA expression levels are significantly elevated in the cortex of P90 Gtf2i-KO mice, as compared to controls. B–K control normalized to 1. Protein levels were normalized to β-tubulin IV and mRNA levels were normalized to Gapdh. Data are presented as mean values ± SEM. B–D, F–K Two-sided t-test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Source data are provided as a Source Data file.
