Table 2 Frequency of selection for mutants with elevated minimum inhibitory concentration (MIC) for three strains of Neisseria gonorrhoeae exposed to increasing concentrations of ciprofloxacin or Debio 1453

From: The bactericidal FabI inhibitor Debio 1453 clears antibiotic-resistant Neisseria gonorrhoeae infection in vivo

Antibiotic

Fold MIC

 

N. gonorrhoeae strain

ATCC 700825a

6926

6804 (CIP-R)

WHO Z (CIP-R, CRO-R)

Ciprofloxacin

4×

6.38 × 10−9

1.50 × 10−9

NT

NT

8×

<6.38 × 10−9

1.88 × 10−9

NT

NT

16×

<6.38 × 10−9

3.75 × 10−10

NT

NT

Debio 1453

4×

<6.38 × 10−9

<9.38 × 10−9

7.06 × 10−9

3.85 × 10−10

1.06 × 10−9

2.81 × 10−10

8×

<6.38 × 10−9

<9.38 × 10−9

7.06 × 10−9

<3.85 × 10−10

<5.28 × 10−10

<2.81 × 10−10

16×

<6.38 × 10−9

<9.38 × 10−9

<7.06 × 10−9

<3.85 × 10−10

<5.28 × 10−10

<2.81 × 10−10

  1. Instances where no mutant colonies were observed are denoted as less than (<) one colony divided by the starting inoculum. NT, not tested as both 6804 and WHO Z are ciprofloxacin-resistant. CIP-R, ciprofloxacin-resistant; CRO-R, ceftriaxone-resistant. Relevant Debio 1453 MICs for this assay were 0.016 μg/mL for ATCC 700825, 0.03 μg/mL for 6926, 0.06 μg/mL for 6804 and 0.125 μg/mL for WHO Z. Source data are provided as a Source Data file.
  2. aATCC 700825 was performed in biological triplicate using an inoculum of ~108 CFU; the remaining strains were performed once using an inoculum of ~109 CFU.
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