Fig. 5: Label-free human sweat profiling of assorted metabolites.
From: All-flexible chronoepifluidic nanoplasmonic patch for label-free metabolite profiling in sweat
a Schematic illustration of on-body evaluation. Optical images of (b) on-body evaluation (left) and the CEP-SERS patch attached to human skin (right), (c) sequential sweat sampling (top), and sample preservation under different physical distortions (bottom). The scale bar represents 10 mm. Accuracy of label-free quantification for (d) uric acid and (e) lactate in the human sweat. f Comparison between tyrosine concentrations depending on participants measured by CMA (light gray) and SERS (dark gray). g−i Chronological profiling (n = 6 technical replicates) of each metabolite during exercise (left) measured by SERS (red rectangles) and FMA (blue circles) presented with schematic illustrations of each metabolic pathway (right). The error bars represent one standard deviation from the mean. j Measured sweat rate (solid line), heart rate (dotted line), and respiratory exchange ratio (RER, yellow bar) during on-body evaluation. Concentration comparison of (k) uric acid and (l) tyrosine under different physiological conditions. (n = 26 for fasting, n = 29 for purine intake, center line, median; box limits, upper and lower quartiles; whiskers, 1.5x interquartile range; cross, outlier; point, data) (m) Relative metabolic phenotyping of individuals (IN, thin solid line) and averaged result (bold solid line) under fasting conditions (blue) and after purine-rich diet intake (red).
