Fig. 2: Autotrophic PHB production by the mechanically-stimulated ZnO-C. necator hybrid system.
a The autotrophic PHB production time course over 48 h by ZnO-C. necator submitted to US at 96 W for 1 h per day. b Bar graph comparing the optimal PHB concentration observed with the US-stimulated hybrid system at 36 h versus PHB production by C. necator alone, submitted or not to US, or by ZnO-C. necator without US (p1 = 1.476 × 10-5, p2 = 1.2360 × 10-5, and p3 = 0.0001). PHB production c time course curves and d bar graph of C. necator grown with 0 to 1 g/L ZnO NSs with US at 96 W for 1 h per day. e PHB production time course curves and f bar graph of C. necator alone or the ZnO-C. necator hybrid system submitted to intense wave motion via tilting agitation at 200 rpm (p1 = 8.5483 × 10-8). g NADPH/NADP+ ratios (p1 = 0.0044, p2 = 0.0140, p3 = 0.0065, and p4 = 0.0015), h viable cell percentages, and i cell densities after 36 h of autotrophic growth of the mechanically stimulated ZnO-C. necator hybrid system and the relevant controls (p1 = 0.0226, p2 = 0.0078, p3 = 0.0018, and p4 = 0.0046). Cn: C. necator. Data are mean ± s.d. (n = 3 independent experiments); two-tailed paired Student’s t-test; *p ≤ 0.05. Source data are provided as a Source Data file.
