Fig. 8: Disruption of Spindle pole-localized F-actin cage leads to chromosome missegregation.

A Time-lapse confocal microscopy of MTOCs (Cep192-mCherry, pseudo gray) and DNA (SiR-DNA, pseudo magenta) in control and spindle-pole F-actin-perturbed oocytes. Prometaphase I mouse oocytes were treated with Optojasp-1 and exposed to 405 nm laser at the cytoplasm (control) or treated with Optojasp-1 and exposed to 405 nm laser at both spindle poles (spindle pole-localized F-actin perturbation). Images were taken every 30 min. Scale bar represents 20 μm. Chromosomes were tracked throughout live imaging by using the autotracking function of Imaris software. Yellow arrowheads represent lagging chromosomes. Time points represent the timing from the start of confocal imaging. B Average percentage of misaligned chromosomes at metaphase I of control and spindle pole F-actin-perturbed oocytes. C Average percentage of lagging chromosomes at anaphase I/telophase I of control and spindle pole F-actin-perturbed oocytes. D Representative images of euploid metaphase II oocytes assessed for aneuploidy by in situ chromosome counting. Quantification of the percentage of aneuploidy in the indicated groups (right panel). Data are represented as means ± SEM. Asterisk represents significant differences, *p < 0.05, **p < 0.01. The total number of analyzed oocytes per each experimental group is specified above each graph, based on 4 independent replicates using 8 mice for panel (D), and 2 independent replicates using 6 mice for panels (B and C).