Fig. 3: The relationship between the extracellular electron transfer (EET) process and the nitrogen removal process.
From: Electron shuttling promotes denitrification and mitigates nitrous oxide emissions in lakes
a Two-sided simple linear regression between standardized EET genes and denitrifying genes. b Two-sided simple linear regression between standardized EET genes and N2O reducing genes. c Two-sided simple linear regression between humic substances and EET gene omcB. d Exponential correlation between the EET functional gene and dissolved N2 characteristics (y = a*exp(b*x)+c). For parameter b, the one-sided t-test results are: t(7) = 2.105, p = 0.0366 for the N2 model; and t(7) = 1.945, p = 0.0464 for the ΔN2 model. All functional genes were standardized by 16S rRNA gene abundance. Dots represent individual sampling sites for n = 12 biological replicates. The dark-red solid lines denote the linear regression fits (a–c). The light gray shading with red borders indicates the 95% confidence intervals (CI) around the regression lines. Statistical significance was determined at p < 0.05.
