Fig. 4: Experimental perturbations of hexamer sequences alter splice-site choice.
From: A basic framework to explain splice-site choice in eukaryotes
A Direct comparison of competing hexamers in minigene constructs of Rosenberg et al.30. Blue line represents permuted thresholds calculated from 10,000 permutations and the actual experimental data (red) with varying combinations of hexamers. The number of unique competing pairs tested is shown above the percentages. Conversion of a good intron into a bad intron (B) or bad intron into a good intron (C) through change of hexamers. mCHERRY fluorescence is shown along with the RT-PCR amplification. The experiments were repeated twice in two independent experiments, which yielded similar results.
