Fig. 6: Piezo1 channels-involved NO signaling pathways.

a Piezo1 (green) and nucleus (blue) immunofluorescence staining of MC3T3-E1 cells. b Normalized Ca²⁺ fluorescence intensity traces (relative to unstimulated cells) of MC3T3-E1 cells in response to the application of Yoda1 (40 μM) and DMSO. c Amperometric responses and (d) the corresponding quantitative analysis of NO molecules detected from MC3T3-E1 cells stimulated by 40 μM Yoda1. The red triangle indicates the reagent addition. Applied potential: +0.80 V (vs. Ag/AgCl). Data are presented as mean ± s.e.m.; two-tailed Student’s t-test (n = 3, for each group). e Normalized peak Ca²⁺ fluorescence intensity (relative to the undeformed cells) of MC3T3-E1 cells induced by 10% magneto-responsive deformation under different conditions. Data are presented as mean ± s.e.m.; one-way ANOVA (n = 6, for each group). f Amperometric responses and (g) the corresponding quantitative analysis of NO molecules detected from MC3T3-E1 cells under different conditions. The vertical gray dashed line indicates the beginning of 10% deformation. Applied potential: +0.80 V (vs. Ag/AgCl). Data are presented as mean ± s.e.m.; one-way ANOVA (n = 3, for each group). h Schematic diagram showing the mechanically activated NO signaling pathways.