Fig. 5: AliA and AliB are important for the growth, cell shape, and motility of Hfx. volcanii.

a Functional classification of 93 predicted Hfx. volcanii lipoproteins. PTM, posttranslational modification. b Colony morphology of Hfx. volcanii wild type and ∆ali mutants. Strains were streaked out on Hv-Cab agar plates and incubated at 45 °C for 4 days prior to imaging. c Growth curves of Hfx. volcanii wild type and ∆ali mutants in Hv-Cab medium. Data are presented as mean values of four biological replicates +/− standard deviations. d Growth curves of Hfx. volcanii wild type and ∆ali mutants in minimal medium with glucose and alanine as the sole carbon and nitrogen sources, respectively. Data are presented as mean values of four biological replicates +/− standard deviations. e Cell morphology of Hfx. volcanii wild type and ∆ali mutants in early log and the quantification. Cells with aspect ratios (ratio of major to minor axes) <2 are considered disks and short rods, while others are considered regular rods. Three biological replicates were analyzed for each strain, and 257 cells were analyzed for each biological replicate. Scale bars indicate 5 µm. Data were analyzed using a one-way ANOVA test. ****P < 0.0001. f Motility halos of Hfx. volcanii wild type and ∆ali mutants and the quantification. Scale bars indicate 0.5 cm. The quantification shows the motility halo diameters and their mean of five biological replicates for each strain. Data were analyzed using a one-way ANOVA test. Error bars represent standard deviations. ****P < 0.0001.