Fig. 5: Storage temperatures and buffer choice impact siRNA-LNP knockdown. | Nature Communications

Fig. 5: Storage temperatures and buffer choice impact siRNA-LNP knockdown.

From: Buffer optimization of siRNA-lipid nanoparticles mitigates lipid oxidation and RNA-lipid adduct formation

Fig. 5: Storage temperatures and buffer choice impact siRNA-LNP knockdown.The alternative text for this image may have been generated using AI.

A Relative mRNA expression was measured in response to increasing sample concentration (n = 2 independent replicates), normalized to an untreated control. Except for fresh LNP formulated on the day of assay, all LNP samples were stored at 2-8 °C or 25 °C for four weeks in 1X PBS or 10 mM histidine buffer. siHPRT incubated with RNAiMax and free siHPRT were used as positive and negative controls, respectively. Markers and error bars represent the mean and standard deviation between experimental duplicates. At 0.1 nM siRNA, HPRT expression was compared across groups to fresh LNPs using a two-tailed Student’s t-test assuming unequal sample variance. LNPs stored in PBS at refrigerated temperature, or LNPs stored in histidine buffer at either refrigerated or room temperature, showed no significant difference (p-values > 0.05), while LNPs at room temperature in PBS resulted in negligible knockdown (p = 0.02). B IC50 values for the samples were determined using nonlinear regression. N/A refers to samples for which a plateau was not established over the tested concentrations. Source data are provided as a Source Data file.

Back to article page