Fig. 3: BRN3B tunes morphological properties of ipRGC subtypes.
From: Genetic tuning of retinal ganglion cell subtype identity to drive visual behavior
A Representative dendritic arbor tracing of M4 and M2 ipRGCs in control (Opn4Cre/+; Brn3b+/+) and Brn3bcKO mice. B Subtype identified by presence (M4) or absence (M2) of SMI-32 immunolabeling (dashed ellipses), examples are from Brn3bcKO cells. C (Top) Scheme of retrogradely labeling strategy of M1 ipRGCs. (Bottom) Representative dendritic arbor tracing of M1 ipRGCs in control and Brn3bcKO mice. D Sholl analysis of M4 (n = 30 cells in Control, n = 23 cells in Brn3bcKO), M2 (n = 5 cells per group) and M1 (n = 36 cells in Control, n = 18 cells in Brn3bcKO) ipRGC subtypes. ipRGCs from Brn3bcKO (green) mice present less complex dendritic arbors compared to control (black) mice. E, F Brn3bcKO showed decreased dendritic field diameter (E) (n = 30 cells in Control M4, n = 23 cells in Brn3bcKO M4, P = 0.013; n = 5 cells in M2 Control and Brn3bcKO, P = 0.028; and Brn3bcKO, n = 36 cells in Control M1 and n = 18 cells in Brn3bcKO M1, P = 0.0004) and soma size (F) (n = 30 cells in Control M4, n = 23 cells in Brn3bcKO M4, P = 0.016; n = 24 cells in Control M2, n = 32 cells in Brn3bcKO, P = 0.0001; and Brn3bcKO, n = 36 cells in Control M1 and n = 19 cells in Brn3bcKO M1, P = 0.0004) in M4, M2 and M1 ipRGC subtypes than control mice. Source data are provided as a Source Data file. All data are Mean ± SEM, *P < 0.05, ***P < 0.001, two-tailed Student’s t- and Mann Whitney U tests.
